ZFIN ID: ZDB-FIG-070917-121
Ishimaru et al., 2005 - Two families of candidate taste receptors in fishes. Mechanisms of Development   122(12):1310-1321 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 3 Expression of zfT1R and zfT2R genes in gustatory tissues. Tissues containing taste buds were sectioned, and hybridized with digoxigenin-labeled riboprobes for each of the zfT1Rs and zfT2Rs as indicated on the left. The transcripts of zfT1R1, zfT1R2a, zfT1R2b, zfT1R3, zfT2R1a, and zfT2R1b were detected in the taste buds of the lips, gill rakers, and pharynx. The frequencies of zfT1R- and zfT2R-positive cells were lower than that of zfPLC-β2-positive cells. No signals for zfT1Rs or zfT2Rs were detected in the olfactory epithelium, while signals for zf5.24, an ortholog of goldfish olfactory receptor 5.24 gene, were detected in the adjacent section. The black label in olfactory epithelium hybridized with probe for zfT2R1a is a cluster of endogenous pigments. Scale bars at the bottom of each column=10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
plcb2 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
Adult taste bud ISH
tas1r1 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
tas1r2.1 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
tas1r2.2 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
tas1r3 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
tas2r200.1 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
tas2r200.2 WT standard conditions Adult gill raker ISH
Adult mouth ISH
Adult pharynx ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Mechanisms of Development, 122(12), Ishimaru, Y., Okada, S., Naito, H., Nagai, T., Yasuoka, A., Matsumoto, I., and Abe, K., Two families of candidate taste receptors in fishes, 1310-1321, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.