ZFIN ID: ZDB-FIG-070905-7
Yin et al., 2007 - Convergence and extension movements mediate the specification and fate maintenance of zebrafish slow muscle precursors. Developmental Biology   304(1):141-155 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 1 Loss of Kny and Tri function impairs zebrafish slow muscle development. (A–D) Confocal images of dorsal views of embryos expressing mGFP. Red lines delineate the outline of the 5th somite. (E–H) Trunk region of day 1-embryos expressing mGFP, lateral views. (I–L) Dorsal views showing smbpc RNA expression in the slow muscles. (M–P) Lateral views of embryos stained with F59 antibody (green) that labels the cytoplasm of the slow muscle cells and Prox1 antibody (red) that labels their nuclei. A, anterior. P, posterior. NC, notochord. Scale bars: (A–H, M–P), 50 μm. (I–L), 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mybpc1 WT standard conditions Prim-5 slow muscle cell ISH
gpc4m119/m119 standard conditions Prim-5 slow muscle cell ISH
gpc4m119/m119; vangl2m209/m209 standard conditions Prim-5 slow muscle cell ISH
vangl2m209/m209 standard conditions Prim-5 slow muscle cell ISH
prox1a WT standard conditions Prim-5 slow muscle cell IHC
gpc4m119/m119 standard conditions Prim-5 slow muscle cell IHC
gpc4m119/m119; vangl2m209/m209 standard conditions Prim-5 slow muscle cell IHC
vangl2m209/m209 standard conditions Prim-5 slow muscle cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4m119/m119 standard conditions 5-9 somites somite decreased length, abnormal
5-9 somites somite increased width, abnormal
Prim-5 myotome decreased length, abnormal
gpc4m119/m119; vangl2m209/m209 standard conditions 5-9 somites somite decreased length, abnormal
gpc4m119/m119; vangl2m209/m209 standard conditions 5-9 somites somite increased width, abnormal
gpc4m119/m119; vangl2m209/m209 standard conditions Prim-5 myotome disorganized, abnormal
gpc4m119/m119; vangl2m209/m209 standard conditions Prim-5 slow muscle cell decreased amount, abnormal
gpc4m119/m119; vangl2m209/m209 standard conditions Prim-5 slow muscle cell disorganized, abnormal
gpc4m119/m119; vangl2m209/m209 standard conditions Prim-5 slow muscle cell disoriented, abnormal
vangl2m209/m209 standard conditions 5-9 somites somite decreased length, abnormal
5-9 somites somite increased width, abnormal
Prim-5 myotome decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 304(1), Yin, C., and Solnica-Krezel, L., Convergence and extension movements mediate the specification and fate maintenance of zebrafish slow muscle precursors, 141-155, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.