ZFIN ID: ZDB-FIG-070813-21
Wendl et al., 2007 - Early developmental specification of the thyroid gland depends on han-expressing surrounding tissue and on FGF signals. Development (Cambridge, England)   134(15):2871-2879 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 5 Elimination of han expression sites in the pharyngeal area does not influence thyroid development. Labelling is as in Fig. 1. (A-D) In edn1 morphants (B), han (hand2) expression in the first (1) and third branchial arches is eliminated. In tfap2a(low, lockjaw) morphants (C), hand2 expression in the second branchial arch (2) is missing. In double morphants (D), hand2-expressing cells are only present in arch-associated cells (arrowheads), as well as some weak expression in the endoderm (confirmed on sections, data not shown). (E) The thyroid (arrow) appears normal in edn1/tfap2a double morphants. (F) In foxi1 morphants, hand2 expression in arch-associated cells (arrowhead) is specifically ablated. (G) Normal expression of nk2.1a in foxi1 morphants (arrow). h, heart.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hand2 WT control Prim-5 heart tube ISH
Prim-5 pharyngeal arch ISH
WT + MO1-edn1 standard conditions Prim-5 endoderm ISH
Prim-5 heart tube ISH
Prim-5 Not Detected pharyngeal arch 3 skeleton ISH
Prim-5 Not Detected pharyngeal arch 5 skeleton ISH
WT + MO1-edn1 + MO2-tfap2a standard conditions Prim-5 endoderm ISH
Prim-5 heart tube ISH
Prim-5 Not Detected pharyngeal arch 3 skeleton ISH
Prim-5 Not Detected pharyngeal arch 4 skeleton ISH
Prim-5 Not Detected pharyngeal arch 5 skeleton ISH
WT + MO1-foxi1 standard conditions Prim-5 heart tube ISH
Prim-5 pharyngeal arch ISH
WT + MO2-tfap2a standard conditions Prim-5 endoderm ISH
Prim-5 heart tube ISH
Prim-5 Not Detected pharyngeal arch 4 skeleton ISH
nkx2.4b WT + MO1-foxi1 standard conditions Long-pec thyroid follicle ISH
slc5a5 WT + MO1-edn1 + MO2-tfap2a standard conditions Pec-fin thyroid follicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-edn1 + MO2-tfap2a standard conditions Pec-fin thyroid follicle morphology, normal
WT + MO1-foxi1 standard conditions Long-pec thyroid follicle morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development