Fig. S4

fgf3+8 morphants phenocopy fgf3/8 double mutant phenotype. Zebrafish embryos injected with a fgf3 and fgf8 morpholino alone or together and uninjected controls were collected at 36 hpf and processed for in situ hybridization with phox2b riboprobe. All panels show lateral views. Notice that injection of the suboptimal amounts of fgf3-MO alone causes only a slight reduction in the EB ganglia. However, injection of the same amount of fgf3-MO together with fgf8-MO causes complete absence of the EB ganglia. Also notice that the locus coeruleus is completely eliminated in fgf3+8 morphants, allowing the efficiency of injections to be monitored. a, acoustic ganglion; al, anterior lateral line ganglion; f, facial placode or ganglion; g, glossopharyngeal placode or ganglion; mhb, midhindbrain boundary; nt, neural tube; ov, otic vesicle; pl, posterior lateral line ganglion; t, trigeminal ganglion; v, vagal placode or ganglion; lc, locus coeruleus; nc, vagal neural crest. Scale bars, 50 μm.