FIGURE

Fig. 4

ID

ZDB-FIG-070314-34

Publication

Songhet et al., 2007 - fgf1 is required for normal differentiation of erythrocytes in zebrafish primitive hematopoiesis

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Fig. 4

fgf1 morphants have persistent high levels of gata1 expression at 35 hours postfertilization (hpf). The 35 polymerase chain reaction (PCR) cycles with gata1-specific primers amplify visibly less product from control morphants than from fgf1 morphants at 35 hpf (standard reverse transcriptase-quantitative PCR). Comparison to 24 hpf control and fgf1 morphants indicates continuously high gata1 expression in fgf1 morphants, but a down-regulation in control morphants, confirming the in situ hybridization data. bactin2 (beta actin2) expression is used as control. Identical results were observed in three independent sets of experiments.

Expression Data

Gene:	gata1a
Fish:	WT WT + MO1-fgf1a + MO2-fgf1a
Knockdown Reagents:	MO1-fgf1a MO2-fgf1a
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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