Fig. 4
- ID
- ZDB-IMAGE-070314-28
- Genes
- Publication
- Songhet et al., 2007 - fgf1 is required for normal differentiation of erythrocytes in zebrafish primitive hematopoiesis
- All Figures
- Figures for Songhet et al., 2007
Fig. 4 fgf1 morphants have persistent high levels of gata1 expression at 35 hours postfertilization (hpf). The 35 polymerase chain reaction (PCR) cycles with gata1-specific primers amplify visibly less product from control morphants than from fgf1 morphants at 35 hpf (standard reverse transcriptase-quantitative PCR). Comparison to 24 hpf control and fgf1 morphants indicates continuously high gata1 expression in fgf1 morphants, but a down-regulation in control morphants, confirming the in situ hybridization data. bactin2 (beta actin2) expression is used as control. Identical results were observed in three independent sets of experiments.