FIGURE

Fig. 7

ID

ZDB-FIG-070314-3

Publication

Panizzi et al., 2007 - New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia

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Fig. 7

Apical-basal distribution of Na⁺/K⁺-ATPase is disrupted in morphants. Cryosections through pronephric ducts of uninjected WT (A,C,E,G) and MO^AUG-injected (B,D,F,H) embryos at the indicated developmental times. The lumen (apical) is indicated by an asterisk, and the basal sides of ducts are outlined by dotted lines in A-F. (A,B) Filamentous actin in the pronephric ducts (red). (C,D) Cell junctions are stained with ZO1 antibody (red, arrowheads), and the apical region of the cells is marked by aPKCζ (green). (E,F) Microtubule-organizing centers are stained with anti-γ-tubulin (red, arrowheads), and the apical region of the cells is marked by aPKCζ (green). (G,H) Localization of Na⁺/K⁺-ATPase (red); the apical region of the cells is marked by PKCζ (green). Arrows highlight the presence of staining in basal regions of WT control cells and its absence in morphants. Scale bar: 5 μm.

Expression Data

Genes:	prkci tjp1a
Fish:	WT WT + MO1-arhgef11
Knockdown Reagent:	MO1-arhgef11
Anatomical Term:	pronephric duct
Stage Range:	Prim-5 to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-arhgef11
Knockdown Reagent:	MO1-arhgef11
Observed In:	pronephric duct actin cytoskeleton pronephric duct sodium:potassium-exchanging ATPase complex
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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