ZFIN ID: ZDB-FIG-070314-3
Panizzi et al., 2007 - New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia. Development (Cambridge, England)   134(5):921-931 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 7 Apical-basal distribution of Na+/K+-ATPase is disrupted in morphants. Cryosections through pronephric ducts of uninjected WT (A,C,E,G) and MOAUG-injected (B,D,F,H) embryos at the indicated developmental times. The lumen (apical) is indicated by an asterisk, and the basal sides of ducts are outlined by dotted lines in A-F. (A,B) Filamentous actin in the pronephric ducts (red). (C,D) Cell junctions are stained with ZO1 antibody (red, arrowheads), and the apical region of the cells is marked by aPKCζ (green). (E,F) Microtubule-organizing centers are stained with anti-γ-tubulin (red, arrowheads), and the apical region of the cells is marked by aPKCζ (green). (G,H) Localization of Na+/K+-ATPase (red); the apical region of the cells is marked by PKCζ (green). Arrows highlight the presence of staining in basal regions of WT control cells and its absence in morphants. Scale bar: 5 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prkci WT standard conditions Prim-5 pronephric duct IHC
Long-pec pronephric duct IHC
Pec-fin pronephric duct IHC
WT + MO1-arhgef11 standard conditions Prim-5 pronephric duct IHC
Long-pec pronephric duct IHC
Pec-fin pronephric duct IHC
tjp1a WT standard conditions Pec-fin pronephric duct IHC
WT + MO1-arhgef11 standard conditions Pec-fin pronephric duct IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-arhgef11 standard conditions Prim-5 pronephric duct actin cytoskeleton disorganized, abnormal
Long-pec pronephric duct sodium:potassium-exchanging ATPase complex mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development