FIGURE

Fig. 4

ID

ZDB-FIG-070312-7

Publication

Panizzi et al., 2007 - New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia

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Fig. 4

Arhgef11 is not required for formation of Kupffer′s vesicle and cilia. (A,B) Posterior region of live, uninjected WT and MO-injected embryos. The inset is a magnified view of Kupffer′s vesicle. White scale bar, 100 μm; black scale bar, 25 μm. (C,D) Acetylated tubulin immunostaining of cilia within Kupffer′s vesicle. Scale bar, 10 μm. (E) Comparison of Kupffer′s vesicle cilia length for WT control, MO-injected (AUG, SPL) and vu7/vu7 embryos. The asterisk marks significantly different lengths (P≤0.001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-arhgef11 WT + MO2-arhgef11 Df(Chr07:vangl2,arhgef11)vu7/vu7
Knockdown Reagents:	MO1-arhgef11 MO2-arhgef11
Observed In:	Kupffer's vesicle Kupffer's vesicle cilium neural tube notochord
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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