Fig. 2

Interference with Arhgef11 function yields morphological defects in zebrafish embryos. (A) Western blot using α-zRG on extracts prepared at 13 hpf from the indicated embryos. The asterisk marks expression of an unidentified 85 kDa protein also detected by α-zRG. (B-F) Live embryos at 32 hpf: uninjected WT (B), WT injected with 4 ng MO^AUG (AUGMO; C), WT injected with 350 pg of RNA encoding ΔDHPH construct (D), vu7/vu7 mutants (E) and WT injected with 5 ng MO^SPL (SPLMO) (F). Scale bar, 300 μm. (G) Schematic depicting the MO^SPL-binding site (red) at the exon 10-intron 10 boundary, with normal (solid line) and disrupted (dotted line) splicing shown. (H) Ethidium bromide stained agarose gel of RT-PCR products amplified from embryos after the indicated treatment using the primers represented by the blue arrows in G. (I) Western blot using α-zRG on extracts prepared at 13 hpf from uninjected WT, or WT after injection with 4 ng MO^AUG, 5 ng MO^SPL, or co-injected with both.