ZFIN ID: ZDB-FIG-070312-5
Panizzi et al., 2007 - New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia. Development (Cambridge, England)   134(5):921-931 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 2 Interference with Arhgef11 function yields morphological defects in zebrafish embryos. (A) Western blot using α-zRG on extracts prepared at 13 hpf from the indicated embryos. The asterisk marks expression of an unidentified 85 kDa protein also detected by α-zRG. (B-F) Live embryos at 32 hpf: uninjected WT (B), WT injected with 4 ng MOAUG (AUGMO; C), WT injected with 350 pg of RNA encoding ΔDHPH construct (D), vu7/vu7 mutants (E) and WT injected with 5 ng MOSPL (SPLMO) (F). Scale bar, 300 μm. (G) Schematic depicting the MOSPL-binding site (red) at the exon 10-intron 10 boundary, with normal (solid line) and disrupted (dotted line) splicing shown. (H) Ethidium bromide stained agarose gel of RT-PCR products amplified from embryos after the indicated treatment using the primers represented by the blue arrows in G. (I) Western blot using α-zRG on extracts prepared at 13 hpf from uninjected WT, or WT after injection with 4 ng MOAUG, 5 ng MOSPL, or co-injected with both.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arhgef11 WT standard conditions 5-9 somites whole organism WB
WT + MO1-arhgef11 standard conditions 5-9 somites whole organism WB
WT + MO1-arhgef11 + MO2-arhgef11 standard conditions 5-9 somites Not Detected whole organism WB
WT + MO2-arhgef11 standard conditions 5-9 somites whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
Df(Chr07:stbm)vu7/vu7 standard conditions Prim-15 trunk curved ventral, abnormal
Prim-15 whole organism decreased length, abnormal
Long-pec brain development disrupted, abnormal
Long-pec pericardium edematous, abnormal
Long-pec pronephros distended, abnormal
WT + MO1-arhgef11 standard conditions Prim-15 trunk curved ventral, abnormal
Prim-15 ventricular system increased size, abnormal
WT + MO2-arhgef11 standard conditions Prim-15 trunk curved ventral, abnormal
Prim-15 ventricular system increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development