ZFIN ID: ZDB-FIG-070219-23
Millimaki et al., 2007 - Zebrafish atoh1 genes: classic proneural activity in the inner ear and regulation by Fgf and Notch. Development (Cambridge, England)   134(2):295-305 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-4 somites to 20-25 somites
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: 20-25 somites

Fig. 6 Heat-shock induction of NICD. (A-D) Expression of atoh1b and ngn1 at 11 hpf (A,B) and 12 hpf (C,D) in control embryos (A,C) or NICD-positive embryos (B,D) heat shocked at 9 hpf. Loss of ngn1 expression, which is non-overlapping with atoh1b, confirms effective NICD-induction. (E-H) Expression of atoh1a at 19 hpf (E,F) and 20 hpf (G,H) in atoh1b morphants without NICD (E,G) or with NICD (F,H) heat shocked at 18 hpf. Otic vesicles are outlined. Arrowheads mark otic expression domains. All are lateral views with anterior to the left. Scale bar: 15 μm. tg, trigeminal ganglion.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1a WT + MO1-atoh1b heat shock 20-25 somites Not Detected otic vesicle ISH
20-25 somites otic vesicle ISH
kca3Tg/+; kca4Tg/+ + MO1-atoh1b heat shock 20-25 somites Not Detected otic vesicle ISH
20-25 somites otic vesicle ISH
atoh1b WT heat shock 1-4 somites otic placode ISH
5-9 somites otic placode ISH
kca3Tg/+; kca4Tg/+ heat shock 1-4 somites otic placode ISH
5-9 somites Not Detected otic placode ISH
neurog1 WT heat shock 1-4 somites trigeminal placode ISH
kca3Tg/+; kca4Tg/+ heat shock 1-4 somites Not Detected trigeminal placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg/+; kca4Tg/+ + MO1-atoh1b heat shock 20-25 somites hair cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development