| ZFIN ID: ZDB-FIG-070219-23 |
Millimaki et al., 2007
- Zebrafish atoh1 genes: classic proneural activity in the inner ear and regulation by Fgf and Notch.
Development (Cambridge, England)
134(2):295-305
Full text @ Development
EXPRESSION / LABELING:
| Genes: | |
|---|---|
| Fish: | |
| Condition: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | 1-4 somites to 20-25 somites |
PHENOTYPE:
| Fish: | |
|---|---|
| Condition: | |
| Knockdown Reagent: | |
| Observed In: | |
| Stage: | 20-25 somites |
Fig. 6 Heat-shock induction of NICD. (A-D) Expression of atoh1b and ngn1 at 11 hpf (A,B) and 12 hpf (C,D) in control embryos (A,C) or NICD-positive embryos (B,D) heat shocked at 9 hpf. Loss of ngn1 expression, which is non-overlapping with atoh1b, confirms effective NICD-induction. (E-H) Expression of atoh1a at 19 hpf (E,F) and 20 hpf (G,H) in atoh1b morphants without NICD (E,G) or with NICD (F,H) heat shocked at 18 hpf. Otic vesicles are outlined. Arrowheads mark otic expression domains. All are lateral views with anterior to the left. Scale bar: 15 μm. tg, trigeminal ganglion. |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
|---|---|---|---|---|---|---|---|
| atoh1a | WT + MO1-atoh1b | heat shock | 20-25 somites | Not Detected | otic vesicle | ISH | |
| 20-25 somites | otic vesicle | ISH | |||||
| kca3Tg/+; kca4Tg/+ + MO1-atoh1b | heat shock | 20-25 somites | Not Detected | otic vesicle | ISH | ||
| 20-25 somites | otic vesicle | ISH | |||||
| atoh1b | WT | heat shock | 1-4 somites | otic placode | ISH | ||
| 5-9 somites | otic placode | ISH | |||||
| kca3Tg/+; kca4Tg/+ | heat shock | 1-4 somites | otic placode | ISH | |||
| 5-9 somites | Not Detected | otic placode | ISH | ||||
| neurog1 | WT | heat shock | 1-4 somites | trigeminal placode | ISH | ||
| kca3Tg/+; kca4Tg/+ | heat shock | 1-4 somites | Not Detected | trigeminal placode | ISH |
Antibody Labeling Details
No data available
Phenotype Details
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| kca3Tg/+; kca4Tg/+ + MO1-atoh1b | heat shock | 20-25 somites | hair cell absent, abnormal |
| Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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