ZFIN ID: ZDB-FIG-070214-5
Zecchin et al., 2007 - Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish. Developmental Biology   301(1):192-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-15

Fig. 8  Expression of insulin, somatostatin (PPSS2), glucagon, pax6.2 and pdx1 in ins:Gal4;UAS:NICD bi-genic embryos. Mono-genic control embryos (ins:Gal4 neg and UAS:NICD pos) are on the left and bi-genic embryos (ins:Gal4 pos and UAS:NICD pos) are on the right. Expression of NICD under the control of the insulin promoter leads to the lack of insulin-producing cells but somatostatin and glucagon producing cells are present similarly to control. Genes whose expression is analyzed are indicated on the left while the genotypes are indicated on the top. Embryos are in ventral views with anterior to the left. Individual embryos were genotyped by PCR for presence or absence of each transgene. Scale bar is 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga kca3Tg/+; m1080Tg/+ standard conditions Prim-15 pancreatic bud ISH
kca3Tg/+; m1080Tg/m1080Tg standard conditions Prim-15 pancreatic bud ISH
ins kca3Tg/+; m1080Tg/+ standard conditions 20-25 somites pancreas primordium ISH
Prim-15 pancreatic bud ISH
kca3Tg/+; m1080Tg/m1080Tg standard conditions 20-25 somites pancreas primordium ISH
Prim-15 pancreatic bud ISH
pax6b kca3Tg/+; m1080Tg/+ standard conditions Prim-15 pancreatic bud ISH
kca3Tg/+; m1080Tg/m1080Tg standard conditions Prim-15 pancreatic bud ISH
pdx1 kca3Tg/+; m1080Tg/+ standard conditions Prim-15 pancreatic bud ISH
kca3Tg/+; m1080Tg/m1080Tg standard conditions Prim-15 pancreatic bud ISH
sst2 kca3Tg/+; m1080Tg/+ standard conditions Prim-15 pancreatic bud ISH
kca3Tg/+; m1080Tg/m1080Tg standard conditions Prim-15 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 301(1), Zecchin, E., Filippi, A., Biemar, F., Tiso, N., Pauls, S., Ellertsdottir, E., Gnugge, L., Bortolussi, M., Driever, W., and Argenton, F., Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish, 192-204, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.