Loss-of-Mrdr function results in down-regulation of early bmp expression. Expression profiles of bmp2b (Left), bmp4 (Center Left), bmp7 (Center Right), and chordin (Right) in uninjected (A?D) and rdrDN-injected (E?H) early gastrulae (50% epiboly), and in uninjected (I?L) and injected (M?P) embryos at indicated stages of initiation of gene expression. (M, N, and P) rdratgMO-injected and (O) rdrDN-injected are shown. Arrowheads in A and E point to the dorsal (shield) territory of bmp2b expression (24), which, in contrast to the ventrolateral territory, was not affected by rdrDN. Arrowheads in D and H indicate the limits of chordin expression domain. A?H and I?P are animal pole and lateral views, respectively, dorsal to the right.
|