Prdc expression in the pharyngeal arches. A-D: Prdc expression in the pharyngeal arches between 17 and 24 hours postfertilization (hpf) first in arches 1 and 2 and then 3. E-H: prdc and dlx2 expression at 26 hpf. E,F and G,H depict dorsal and lateral views of embryos stained with prdc and dlx2 probes, respectively. I-K: Cryosections (placed in order from anterior [I] to posterior [K] direction) of double-stained embryos with prdc (blue) and dlx2 (red) riboprobes. L-R: prdc and dlx2 expression at 30 hpf. L,M and N,O dorsal and lateral views of prdc and dlx2 whole-mount staining respectively. P-R: Consecutive cryosections (placed in order from anterior [P] to posterior [R]) of double-stained embryos with prdc (blue) and dlx2 (red) riboprobes. S-Y: prdc and dlx2 expression at 36 hpf. S,T and U,V dorsal and lateral views of prdc and dlx2 whole-mount staining, respectively. W-Y: Cryosections (placed in order from anterior [W] to posterior [Y]) of double-stained embryos with prdc (blue) and dlx2 (red) riboprobes. Z-AF: prdc and dlx2 expression at 42 hpf. Z,AA dorsal and lateral views of prdc expression, respectively. Lateral views of dlx2 (AB) and nkx2.3 (AC) expression. AD-AF: Consecutive cryosections (placed in order from anterior [AD] to posterior [AF]) of double-stained embryos with prdc (blue) and dlx2 (red) riboprobes. AG-AI: Dorsal views of 42 hpf embryos hybridized with dlx2, prdc, and nkx2.3 riboprobes. The lines marked a,b depict the planes of plastic sections shown in the adjacent panels. AJ-AO: Plastic cross-sections of dlx2, prdc, and nkx2.3-stained 42 hpf embryos at the level marked with line a (AJ-AL) and line b (AM-AO) in AH. Black arrows point to dlx2 (AJ,AM) and nkx2.3 expression domains (AL,AO) in pharyngeal arch neural crest and endodermal pouches, respectively. Anterior side in whole-mount images is to the left except in AG-AI to the top. h, hours postfertilization.
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