FIGURE

Fig. 2

ID

ZDB-FIG-060913-6

Publication

Appel et al., 2003 - lunatic fringe regulates delta-notch induction of hypochord in zebrafish

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Fig. 2

lfng is required for hypochord development. A: Lateral view, dorsal up, of 24 hours postfertilization (hpf) embryo injected with lfng 4 ng of mismatch control morpholino oligonucleotides (MO). Floor plate (fp), notochord (nc), and hypochord (hc) developed normally. B: A 24 hpf embryo injected with approximately 4 ng of lfng MO had a deficit of hypochord cells. Floor plate and notochord appeared normal. C: Dorsal view, anterior up, of yolk plug closure (YPC) stage embryo injected with standard control MO and probed for her4 expression, which marks presumptive hypochord precursors (arrows), and pax2, to provide an internal hybridization control (asterisks). D: her4 expression was absent (arrows) in embryo injected with approximately 4 ng of lfng MO, whereas pax2 expression was normal (asterisks). E-G: ntl, twhh, and myoD expression in lfng MO injected embryos. Scale bar in G = 20 μm in A,B, 160 μm in C-G.

Expression Data

Genes:	col2a1a her4.1 myod1 pax2a shhb tbxta
Fish:	WT + MO1-lfng
Knockdown Reagent:	MO1-lfng
Anatomical Terms:	axial chorda mesoderm floor plate midbrain hindbrain boundary neural plate notochord paraxial mesoderm presumptive floor plate
Stage Range:	Bud to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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