ZFIN Image: Appel et al., 2003, Fig. 2

Image

Figure Caption

Fig. 2 lfng is required for hypochord development. A: Lateral view, dorsal up, of 24 hours postfertilization (hpf) embryo injected with lfng 4 ng of mismatch control morpholino oligonucleotides (MO). Floor plate (fp), notochord (nc), and hypochord (hc) developed normally. B: A 24 hpf embryo injected with approximately 4 ng of lfng MO had a deficit of hypochord cells. Floor plate and notochord appeared normal. C: Dorsal view, anterior up, of yolk plug closure (YPC) stage embryo injected with standard control MO and probed for her4 expression, which marks presumptive hypochord precursors (arrows), and pax2, to provide an internal hybridization control (asterisks). D: her4 expression was absent (arrows) in embryo injected with approximately 4 ng of lfng MO, whereas pax2 expression was normal (asterisks). E-G: ntl, twhh, and myoD expression in lfng MO injected embryos. Scale bar in G = 20 μm in A,B, 160 μm in C-G.

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Expression / Labeling details

Acknowledgments

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