FIGURE

Fig. 3

ID

ZDB-FIG-060828-3

Publication

Eaton et al., 2006 - The zebrafish bHLH PAS transcriptional regulator, single-minded 1 (sim1), is required for isotocin cell development

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Fig. 3

Isotocin cells are located within the sim1 expression domain. Coexpression of sim1 and itnp mRNA was determined by double label in situ hybridization histochemistry (ISHH) in 48-hpf embryos. Dorsal is up and anterior to the left. A, D, G: Bright-field images. C, F, I: The corresponding epifluorescence images. B, E, H: The respective bright field and epifluorescence images merged. A-F: sim1 expression is visualized with purple staining and itnp expression is visualized with red staining (indicated by the white arrowhead). A-C: sim1 is darkly stained, quenching the red itnp staining. D-F: sim1 is lightly stained, revealing the red itnp staining. G-I: The chromogenic staining is reversed such that sim1 expression is visualized with red staining and itnp expression is visualized with purple staining. The locations of the anterior commissure and the post-optic commissure are indicted with black dots. t, telencephalon; m, midbrain; hy, hypothalamus.

Expression Data

Genes:	oxt sim1a
Fish:	WT
Anatomical Terms:	diencephalon hypothalamus midbrain telencephalon
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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