Altered gene expression in dino (dintm84) and mercedes (mestm305) mutants during gastrulation, detected by whole- mount in situ hybridization. Expression of: (A) eve1, at 40% epiboly, animal view, dorsal right; (B) eve1, at 70% epiboly, lateral view, dorsal right; (C) eve1, at tailbud stage, vegetal view, dorsal right – the more pronounced staining reflects the expression of eve1 in a group of cells posterior to the Kupfer’s vesicle (Joly et al., 1993); (D) gata2, at 70% epiboly, animal view, dorsal right; (E) gata2, at 70% epiboly, lateral view, dorsal right – the margin of the blastoderm is indicated by arrowheads; (F) sna1, at 80% epiboly, dorsal view; (G) shh, at 70% epiboly, dorsal view; (H) gsc and eve1, at 70% epiboly, animal view, dorsal right; (J) fkd3, at shield stage, animal view, dorsal right. (K) fkd3, at 70% epiboly, dorsal view.
|