FIGURE

Fig. 5

ID
ZDB-FIG-060102-2
Publication
Vanderlaan et al., 2005 - Gli function is essential for motor neuron induction in zebrafish
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Fig. 5

Gli2 contributes to spinal motor neuron induction. Panels A–D show dorsal views, and panels E–L show lateral views of the hindbrain (E–J) and spinal cord (K and L), with anterior to the left. Asterisks in A–D indicate the position of the nIII and nIV motor neurons in the midbrain (A and B), and their absence (C and D). Arrowheads in A–D indicate the nV neurons in r2. (A and E) In control wild-type embryos, nk2.2 expression (E) and organization of cranial motor neurons (A) are identical to those described previously. (B and F) In gli2 MO-injected wild-type embryos, expression of nk2.2 (F) and development of cranial motor neurons (B) are identical to control embryos. (C and G) In control yotty119 mutants, the patterns of loss of nk2.2 expression (G) and cranial motor neurons (C) are similar to those described previously. (D and H) In gli2 MO-injected yotty119 mutants, nk2.2 expression is significantly restored in anterior rhombomeres and the caudal hindbrain (H, arrowheads). Concomitantly, the number of nV neurons (arrowhead) and nX neurons is significantly increased (D), and the pattern looks similar to that in control wild-type embryos. (I and K) In control dtrte370 mutants, net1a is expressed at low levels in the ventral hindbrain, and at higher levels at rhombomere boundaries (I). In the mutant spinal cord (K), islet antibody-labeled motor neurons (arrowhead) are found in similar numbers to wild-type embryos. (J and L) In gli2 MO-injected dtrte370 mutants, net1a expression in the hindbrain (J) shows no significant change (loss). In contrast, the number of motor neurons in the ventral spinal cord (arrowhead) is slightly but reproducibly reduced in MO-injected mutants (L).

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 282(2), Vanderlaan, G., Tyurina, O.V., Karlstrom, R.O., and Chandrasekhar, A., Gli function is essential for motor neuron induction in zebrafish, 550-570, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.