ZFIN ID: ZDB-FIG-050826-4
Formstone et al., 2005 - Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway. Developmental Biology   282(2):320-335 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: 1-4 somites to 10-13 somites

Fig. 7 Zebrafish Fmi1a and Fmi1b proteins co-operate to control cell movement. (A–C, I, J) 10-somite embryos. (E, F, H, L–N) 3-somite embryos. In all dorsal views, anterior is to the top. In all lateral views, dorsal is to the right. Markers are as indicated, px is pax2.1, k is krox20, papc is paraxial protocadherin. (A–C) F1b-GPS1 morphants exhibit undulation of neuroepithelium and tailbud. (D and K) RT-PCR analysis. Titration of F1b-GPS1 (D) and F1a-LamG2 (K) morpholino. CoMo is F1b-GPS or F1a-LamG2 morpholino but with four nucleotide changes. Molecular markers (bp) are indicated to left of gel. (D) 3 μg/ml F1b-GPS1 morpholino generates approximately 65% wild-type and 35% mis-spliced product. P1/P2 indicates primer pair used in PCR (see Fig. 2A). (K) 5 μg/ml F1a-LamG2 morpholino generates approximately 40% wild-type and 60% mis-spliced product. P2/P3 indicates primer pair used in PCR (see Fig. 2G). (E–I) Double morphant embryos. (E) Injection of 2.4 μg/ml and 3.5 μg/ml CoMos for F1a-GPS1 and F1b-GPS1, respectively. (F) Injection of 2.4 μg/ml F1a-GPS1 and 3.5 μg/ml F1b-GPS1. (G) F1a-GPS1 (3 μg/ml) and F1b-GPS1 (3.5 μg/ml) morphant. (H) F1a-GPS1 (4 μg/ml) and F1b-GPS1 (4 μg/ml) morphant. Black arrowhead: mid-hindbrain pax2 expression; white arrow: papc expression; black arrow: pronephric pax2 expression. (I) F1a-GPS1 (2.4 μg/ml) and F1b-GPS1 (3.5 μg/ml) morphant. View of head, arrow indicates pax2.1 expression in optic stalk. (J) 9-somite embryo. (L–M) Double morphant embryo, (L) F1a-GPS1 (2.4 μg/ml) and F1a-LamG2 (4.5 μg/ml). (M) F1a-LamG2 (4.5 μg/ml) and F1b-GPS1 (3.5 μg/ml).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT + MO1-celsr1b standard conditions 10-13 somites unspecified ISH
myod1 WT + MO1-celsr1a + MO1-celsr1b standard conditions 10-13 somites unspecified ISH
WT + MO1-celsr1b standard conditions 10-13 somites unspecified ISH
pax2a WT + MO1-celsr1a + MO1-celsr1b standard conditions 1-4 somites unspecified ISH
10-13 somites unspecified ISH
WT + MO1-celsr1a + MO3-celsr1a standard conditions 1-4 somites unspecified ISH
WT + MO1-celsr1b standard conditions 10-13 somites unspecified ISH
WT + MO1-celsr1b + MO3-celsr1a standard conditions 1-4 somites unspecified ISH
pcdh8 WT + MO1-celsr1a + MO1-celsr1b standard conditions 1-4 somites unspecified ISH
WT + MO1-celsr1a + MO3-celsr1a standard conditions 1-4 somites unspecified ISH
WT + MO1-celsr1b + MO3-celsr1a standard conditions 1-4 somites unspecified ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 282(2), Formstone, C.J., and Mason, I., Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway, 320-335, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.