ZFIN ID: ZDB-FIG-050825-1
Formstone et al., 2005 - Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway. Developmental Biology   282(2):320-335 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 128-cell to Prim-5

Fig. 1 Phylogenetic relationship and embryonic expression of zFmi1 genes. (A) Phylogenetic relationship of zFmi1a and zFmi1b to other flamingo proteins. Estimated evolutionary distance is proportional to line length and numbers indicate confidence. These indicate that both zFmi1a genes are orthologues of the ancestral Fmi1/Celsr1 gene. Sources of predicted protein sequences: zFmi1a and zFmi1b, this study; chick Fmi1/Celsr1 and Fmi3/Celsr3; murine Celsr1, 2 and 3, GenBank accession numbers NM009886, AF031573, AF427498. (B–S) Temporal and spatial expression of zfmi1a and zfmi1b. (B and O) RT-PCR analysis of zfmi1a and zfmi1b expression, respectively (top panel). MW is indicated. Control is EF1α lower panel). (C–N) Wild-type embryos hybridized for zfmi1a at 30% epiboly (C), 40% epiboly (D), shield (E), 65% epiboly (F), 75% epiboly (G and H), 95% epiboly (I and J), 3 somites (K and L), and 6 somites (M and N). Lateral and dorsal views are as indicated on each plate; dorsal is to right and anterior to top, respectively. (H, L, and N) Dorsal views of flat-mounted embryos; anterior is to the top. (E) Arrow indicates expression of zfmi1a in shield. Arrowhead indicates expression of zfmi1a in lateral germ ring. Inset, lateral view; arrow indicates expression of zfmi1a in shield. (H) Small black arrow indicates enriched zfmi1a expression in axial tissue, larger arrow indicates an isolated lateral cell expressing zfmi1a. Arrowhead indicates expression of zfmi1a in head. (J) Inset, dorsal view of animal pole; arrow indicates loss of zfmi1a expression medially. (L) Arrowhead indicates loss of zfmi1a expression within anterior axial tissue, white arrow indicates expression in eye field and black arrow indicates expression within lateral trunk. (P–S) Expression of zfmi1b at sphere (P), shield (Q), 95% epiboly (R), and 16 somite stages. Abbreviations: E, epiboly; s, number of somites.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
celsr1a WT standard conditions 128-cell whole organism RTPCR
64-cell whole organism RTPCR
Dome whole organism RTPCR
30%-epiboly whole organism ISH
Shield germ ring ISH
Shield shield ISH
Shield whole organism RTPCR
75%-epiboly whole organism ISH
90%-epiboly whole organism ISH
1-4 somites axis ISH
1-4 somites optic primordium ISH
1-4 somites trunk ISH
5-9 somites axis ISH
5-9 somites trunk ISH
Prim-5 whole organism RTPCR
celsr1b WT standard conditions 128-cell whole organism RTPCR
64-cell whole organism RTPCR
Dome whole organism RTPCR
Sphere whole organism ISH
Shield whole organism ISH
Shield whole organism RTPCR
90%-epiboly whole organism ISH
14-19 somites head ISH
14-19 somites whole organism ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 282(2), Formstone, C.J., and Mason, I., Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway, 320-335, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.