ZFIN ID: ZDB-FIG-050429-2
Hernandez-Lagunas et al., 2005 - Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification. Developmental Biology   278(2):347-357 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-4 somites to 14-19 somites

Fig. 2 prdm1 MO injection reduces neural crest and eliminates Rohon–Beard sensory neurons, reproducing the nrd phenotype. (A–C, G–I) 11 hpf dorsal views, anterior is to the left. (D–F) Twenty-four hpf dorsal views, anterior to the left. (J–L) Eighteen hpf lateral views, anterior is to the left. (A–C) In situ hybridization with HuC showing the three lateral stripes of primary neurons: lateral (L), intermediate (I) and medial (M). The neurons in the lateral stripe form RB sensory neurons (arrow in A) and are completely absent in nrd (B). Following prdm1 MO injection (C), there is a loss of RB sensory neurons, reproducing the nrd phenotype (B). (D–F) HNK-1 immunohistochemistry at 24 hpf, showing the characteristically large RB sensory neurons in a wildtype embryo (arrow in D). Both nrd (E) and prdm1 MO-injected embryos (F) lack RB sensory neurons. Consistent with a loss of RB sensory neurons, both nrd embryos (H) and prdm1 MO embryos (I) display a reduction in foxd3 expression at 11 hpf as compared to wildtype (G). This early reduction of neural crest remains in older embryos shown with crestin (J–L). Abbreviations: wt, wildtype; nrd, narrowminded mutant embryos; MO, prdm1 MO; DLF, dorsal lateral fasciculas; Scale bar is 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crestin WT standard conditions 14-19 somites neural crest ISH
WT + MO2-prdm1a standard conditions 14-19 somites neural crest ISH
prdm1am805/m805 standard conditions 14-19 somites neural crest ISH
elavl3 WT standard conditions 1-4 somites primary neuron ISH
1-4 somites Rohon-Beard neuron ISH
WT + MO2-prdm1a standard conditions 1-4 somites primary neuron ISH
prdm1am805/m805 standard conditions 1-4 somites primary neuron ISH
foxd3 WT standard conditions 1-4 somites neural crest ISH
WT + MO2-prdm1a standard conditions 1-4 somites neural crest ISH
prdm1am805/m805 standard conditions 1-4 somites neural crest ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 278(2), Hernandez-Lagunas, L., Choi, I.F., Kaji, T., Simpson, P., Hershey, C., Zhou, Y., Zon, L., Mercola, M., and Artinger, K.B., Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification, 347-357, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.