ZFIN ID: ZDB-FIG-050429-4
Hernandez-Lagunas et al., 2005 - Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification. Developmental Biology   278(2):347-357 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 90%-epiboly to 5-9 somites

Fig. 4 Expression of prdm1 in the nervous system of wildtype and mutant nrd zebrafish embryos. Lateral views (A, B) and dorsal view(C–H) where anterior is to the left, except in A and C where anterior is to the top. (A) At 90% epiboly, expression is observed along the border of the neural plate where neural crest cells and RB sensory neurons are being specified (arrow), more strongly at the anterior-most border. At tailbud stages (B), expression remains high at the neural plate border (arrows). The expression is particularly strong at the anterior border at tail bud stages (C). (D) At 11 hpf, expression is high at the lateral edge of the neural plate (arrow; higher magnification in E), and also is expressed ventrally in the adaxial cells (A) adjacent to the midline. (F) By 13 hpf, the expression at the border of the neural plate remains only in the most posterior region (arrow). (G, H) Analysis of prdm1 expression in nrd mutant embryos at 11 hpf. Low (G) and high (H) magnification images of nrdm805 embryos showing reduced expression of prdm1, especially at the neural plate border (arrowheads in G). A slight reduction in expression is also observed in the ventral adaxial cells (A). Abbreviations: wt, wildtype; nrd, narrowminded mutant embryos; A, adaxial cells. Scale bar is 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prdm1a WT standard conditions 90%-epiboly neural plate ISH
1-4 somites adaxial cell ISH
Bud neural plate ISH
1-4 somites neural plate ISH
5-9 somites adaxial cell ISH
5-9 somites neural plate ISH
prdm1am805/m805 standard conditions 1-4 somites adaxial cell ISH
1-4 somites neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 278(2), Hernandez-Lagunas, L., Choi, I.F., Kaji, T., Simpson, P., Hershey, C., Zhou, Y., Zon, L., Mercola, M., and Artinger, K.B., Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification, 347-357, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.