CRISPR

CRISPR1-cyp4v2b

ID
ZDB-CRISPR-220921-1
Name
CRISPR1-cyp4v2b
Previous Names
  • CRISPR1-cyp4v7
Target
Sequence
5' - CAGATGCTGCGCGCAAACAG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end,
Genome Resources
None
Target Location
Genome Build: GRCz11Chromosome: 14
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
hzu14 cyp4v2b
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Expression
Gene expression in Wild Types + CRISPR1-cyp4v2b
No data available
Phenotype
Phenotype resulting from CRISPR1-cyp4v2b
No data available
Phenotype of all Fish created by or utilizing CRISPR1-cyp4v2b
Phenotype Fish Conditions Figures
whole organism cyp4v2b expression decreased amount, abnormal cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Fig. 1 with image from Gao et al., 2022
photoreceptor outer segment layer decreased thickness, abnormal cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Fig. 2 with image from Gao et al., 2022
retinal pigmented epithelium cell shape, abnormal cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Fig. 3 with image from Gao et al., 2022
retinal pigmented epithelium lipid droplet present, abnormal cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Fig. 4 with image from Gao et al., 2022
whole organism ab2-gnat2 labeling decreased amount, abnormal cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Fig. 2 with image from Gao et al., 2022
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Citations
TSV
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