This diagram represents the result of blood glucose from all trail groups. Data represents the mean + SE of the results for three independent assays. Statistical analysis compares difference of the mean of results between T2DM-P and HC, HC-P and T2DM. a Significant difference of other groups vs. HC. b Significant difference of other groups vs. HC-P. c Significant difference of other groups vs. T2DM. d Significant difference of other groups vs. T2DM-P Difference with p < 0.001 was considered significant. Each group contained 6 zebrafish

A Histopathology evaluation of zebrafish small intestine. Intestinal tissues were stained with H&E and studied by microscopy (× 400 resolution). Healthy control group (HC), Healthy control group supplemented with probiotics (HC-P), Diabetic group (T2DM), Diabetic group supplemented with probiotic (T2DM-P). B Villus length increased slightly in the T2DM-P group following probiotic supplementation compared to T2DM group. C. Villus width was slightly higher in the T2DM group compared to T2DM-P group. Data shows mean ± SE for three independent assays

The relative expression of the intestinal pro-inflammatory cytokines after 10 days probiotic intervention. A The effects of probiotic on mRNA expression of IL-1β were determined by PCR. B The relative expression of TNF-α from all trail groups represent. The expression levels of β-actin were used as a loading control. C The Correlation of IL-1β to blood glucose. D The Correlation of TNF-α vs. blood glucose. Control group (HC), Control + Probiotic (HC-P), Diabetic group (T2DM), Diabetic group with probiotic(T2DM-P) groups. a Significant difference of other groups vs. HC. b Significant difference of other groups vs. HC-P. c Significant difference of other groups vs. T2DM. d Significant difference of other groups vs. T2DM-P The mean difference is significant at p < 0.001