PUBLICATION
Gut micobiota alteration by Lactobacillus rhamnosus reduces pro-inflammatory cytokines and glucose level in the adult model of Zebrafish
- Authors
- Bootorabi, F., Saadat, F., Falak, R., Manouchehri, H., Changizi, R., Mohammadi, H., Safavifar, F., Khorramizadeh, M.R.
- ID
- ZDB-PUB-210811-5
- Date
- 2021
- Source
- BMC research notes 14: 302 (Journal)
- Registered Authors
- Bootorabi, Fatemeh, Changizi, Reza, Khorramizadeh, M.Reza, Mohammadi, Hassan, Saadat, Farshid, Safavifar, Farnaz
- Keywords
- Cytokine, Lactobacillus rhamnosus, Probiotic, Type 2 diabetes mellitus (T2DM), Zebrafish
- MeSH Terms
-
- Animals
- Cytokines
- Diabetes Mellitus, Type 2*/therapy
- Glucose
- Humans
- Infant
- Lacticaseibacillus rhamnosus*
- Male
- Probiotics*/pharmacology
- Zebrafish
- PubMed
- 34372916 Full text @ BMC Res. Notes
Citation
Bootorabi, F., Saadat, F., Falak, R., Manouchehri, H., Changizi, R., Mohammadi, H., Safavifar, F., Khorramizadeh, M.R. (2021) Gut micobiota alteration by Lactobacillus rhamnosus reduces pro-inflammatory cytokines and glucose level in the adult model of Zebrafish. BMC research notes. 14:302.
Abstract
Objective Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient's circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology.
Results In this trial 3-month old male adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After 5 days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p = 0.0001). These results suggest that probiotic intervention decreased the blood glucose level in the T2DM-P group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping