FIGURE SUMMARY
Title

Legumain Regulates Differentiation Fate of Human Bone Marrow Stromal Cells and Is Altered in Postmenopausal Osteoporosis

Authors
Jafari, A., Qanie, D., Andersen, T.L., Zhang, Y., Chen, L., Postert, B., Parsons, S., Ditzel, N., Khosla, S., Johansen, H.T., Kjærsgaard-Andersen, P., Delaisse, J.M., Abdallah, B.M., Hesselson, D., Solberg, R., Kassem, M.
Source
Full text @ Stem Cell Reports

Legumain Inhibits OB Differentiation and Bone Mineralization In Vivo

(A) Conservation of zebrafish lgmn. I, amino acid identity; S, amino acid similarity.

(B) High-resolution melting analysis of pooled lgmn-TALEN and control-injected animals at 3 days post-fertilization (dpf).

(C) qRT-PCR analysis of OB and AD marker genes at 5 dpf. Data represent mean ± SEM of three pools of ten animals. p ≤ 0.05, two-tailed unpaired Student’s t test.

(D and E) Control and lgmn mutant animals at 7 dpf. Scale bars, 500 μM. (D) Bright field and (E) fluorescent alizarin red staining. (E′ and E″) Higher-magnification images of boxed regions. Arrowheads, whole vertebrae stained; arrows, partial vertebrae stained. Scale bars, 100 μM.

(F) Number of alizarin-red-stained vertebrae in lgmn and control animals at 7 dpf. Data represent mean ± SEM, n > 30 for each group. p ≤ 0.05, ∗∗∗p ≤ 0.005 two-tailed unpaired Student’s t test.

(G) Animals were treated with SD-134 (500 μM) or 1% DMSO from 3 to 7 dpf. Number of alizarin-red-stained vertebrae at 7 dpf. Data represent mean ± SEM, n = 12 for each group. ∗∗∗p ≤ 0.005 two-tailed unpaired Student’s t test.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Days 7-13
Acknowledgments
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