IMAGE

Fig. 5

ID
ZDB-IMAGE-180906-8
Genes
Source
Image
Figure Caption

Fig. 5

Legumain Inhibits OB Differentiation and Bone Mineralization In Vivo

(A) Conservation of zebrafish lgmn. I, amino acid identity; S, amino acid similarity.

(B) High-resolution melting analysis of pooled lgmn-TALEN and control-injected animals at 3 days post-fertilization (dpf).

(C) qRT-PCR analysis of OB and AD marker genes at 5 dpf. Data represent mean ± SEM of three pools of ten animals. p ≤ 0.05, two-tailed unpaired Student’s t test.

(D and E) Control and lgmn mutant animals at 7 dpf. Scale bars, 500 μM. (D) Bright field and (E) fluorescent alizarin red staining. (E′ and E″) Higher-magnification images of boxed regions. Arrowheads, whole vertebrae stained; arrows, partial vertebrae stained. Scale bars, 100 μM.

(F) Number of alizarin-red-stained vertebrae in lgmn and control animals at 7 dpf. Data represent mean ± SEM, n > 30 for each group. p ≤ 0.05, ∗∗∗p ≤ 0.005 two-tailed unpaired Student’s t test.

(G) Animals were treated with SD-134 (500 μM) or 1% DMSO from 3 to 7 dpf. Number of alizarin-red-stained vertebrae at 7 dpf. Data represent mean ± SEM, n = 12 for each group. ∗∗∗p ≤ 0.005 two-tailed unpaired Student’s t test.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cell Reports