Transgenic Construct

Tg(isl1a:GAL4-VP16,UAS:EGFP)

ID

ZDB-TGCONSTRCT-090917-1

Previous Names

Tg(isl1:GAL4-VP16,UAS:EGFP)
Tg(isl1:Gal4-VP16,UAS:eGFP)
Tg(isl1:Gal4-VP16,UAS:EGFP)
Tg(sensory:GFP)

Type

engineered_plasmid

Regulatory Regions

isl1a (1)
UAS (1)

Coding Sequences

EGFP (1)
GAL4 (1)
VP16 (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

None

Note

A enhancer the islet 1 gene was used to drive expression of GAL4-VP16, which in turn activated eGFP under the control of fourteen copies of the Gal4 upstream activating sequences (UAS).

Plasmid Map

None

Genomic Features

That Utilize Tg(isl1a:GAL4-VP16,UAS:EGFP) Construct

Genomic Feature	Affected Genomic Regions
zf154Tg

Transgenics

That Utilize Tg(isl1a:GAL4-VP16,UAS:EGFP) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
ext2^{to273b/to273b}; la203Tg; zf154Tg	ext2	Fig. 5 from Wang et al., 2012
ext2^{to273b/to273b}; la203Tg; zf154Tg + MO1-ptprfa + MO1-ptprfb		Fig. 7 from Wang et al., 2012
ext2^{to273b/to273b}; la203Tg; zf154Tg + MO1-ptprfb		Fig. 7 from Wang et al., 2012
ext2^{to273b/to273b}; la203Tg; zf154Tg + MO2-ptprfb		Fig. 7 from Wang et al., 2012
la203Tg; zf154Tg			Fig. 2 from Wang et al., 2012
la203Tg; zf154Tg + MO1-ptprfa + MO1-ptprfb		Fig. 2 from Wang et al., 2012
la203Tg; zf154Tg + MO1-ptprfa + MO2-ptprfa		Fig. 2 from Wang et al., 2012
la203Tg; zf154Tg + MO2-ptprfa + MO2-ptprfb		Fig. 2 from Wang et al., 2012
la203Tg; zf154Tg + MO2-ptprfb		Fig. 2 from Wang et al., 2012
la210Tg; zf154Tg (AB)
mitfa^w2/w2; zf154Tg	mitfa		Fig. 1 from Eijkenboom et al., 2018
mitfa^w2/w2; zf154Tg + MO1-gdap1		3 figures from Eijkenboom et al., 2019
mitfa^w2/w2; zf154Tg + MO1-opa1		4 figures from Eijkenboom et al., 2019
zf154Tg			5 figures from 3 publications
zf154Tg + MO1-srrm4 + MO4-tp53		Fig. 6 from Calarco et al., 2009	Fig. 6 from Calarco et al., 2009
zf154Tg + MO4-tp53			Fig. 6 from Calarco et al., 2009

Sequence Information

Other Construct Pages

None

Citations