ZFIN ID: ZDB-TGCONSTRCT-110131-35
Transgenic Construct Name: Tg2(Xla.Rho:crb2a-HA)
Sequence Ontology ID :
Synonyms: Extra_Secr (1), Tg(Xla.Rho:crb2.2-HA) (1), Tg(Xla.Rho:crb2a.2-HA)

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Note: This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide taken from zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Extra_Secr construct, site directed mutagenesis was used to place a Stop codon just before the transmembrane domain.

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CONSTRUCT COMPONENTS
Coding Sequences: HA (1)
crb2a (1)
Contains: HA (1)
GENOMIC FEATURES THAT UTILIZE Tg2(Xla.Rho:crb2a-HA)
Genomic Feature Affected Genomic Regions    
umz7Tg    
umz8Tg    
umz9Tg    
TRANSGENICS THAT UTILIZE Tg2(Xla.Rho:crb2a-HA)
Fish Affected Genomic Regions Phenotype Gene Expression
slc45a2b4/+; fl1Tg/+; umz7Tg/+ slc45a2
slc45a2b4/+; fl1Tg/+; umz8Tg/+ slc45a2
slc45a2b4/b4; fl1Tg; umz7Tg slc45a2 Fig. 4 with image from Hsu et al., 2010 7 figures with image from Hsu et al., 2010
slc45a2b4/b4; fl1Tg; umz8Tg slc45a2
slc45a2b4/b4; fl1Tg; umz9Tg slc45a2
umz9Tg
SEQUENCE INFORMATIONNo data available
OTHER Tg2(Xla.Rho:crb2a-HA) TRANSGENIC CONSTRUCT PAGESNo links to external sites
CITATIONS (5)