Fig. 4

Measurement of rod size in Crb2a transgenics. (A) The average combined volume of rod cell body and inner segment (black circles) measured 332 ± 30 μm³ (wildtype), 372 ± 13.6 μm³ (Crb2a^IntraWT), 394 ± 12.2 μm³ (Crb2a^IntraΔFBD), 416 ± 28.9 μm³ (Crb2a^IntraΔPBD), and 436 ± 22.8 μm³ (Crb2a^{IntraΔFBDΔPBD}). The average volume of a rod outer segment (red circles) measured 190 ± 15.7 μm³ (wildtypes), 277 ± 15.4 μm³ (Crb2a^IntraWT), 283 ± 20.6 μm³ (Crb2a^IntraΔFBD), 278 ± 15.9 μm³ (Crb2a^IntraΔPBD), and 212 ± 18.9 μm³ (Crb2a^{IntraΔFBDΔPBD}). (B) The average combined volume of rod cell body and inner segment (black circles) measured 332 ± 30 μm³ (wildtype), 426 ± 17.5 μm³ (Crb2a^FL), 401 ± 17.8 μm³ (Crb2a^Extra_TM), and 405 ± 11 μm³ (Crb2a^Extra_Secr). The average volume of a rod outer segment (red circles) measured 190 ± 15.7 μm³ (wildtype), 198 ± 13.8 μm³ (Crb2a^FL), 316 ± 17.2 μm³ (Crb2a^Extra_TM), and 268 ± 12.1 μm³ (Crb2a^Extra_Secr). (C, C′) Width of the inner segment myoid region was marked as in (C) and the measurements are shown in (C′). The width of the myoid region is significantly larger in Crb2a^FL rods (2.2 ± 0.1 μm) than wildtypes (1.5 ± 0.08 μm). Crb2a^Extra_TM (1.5 ± 0.07 μm) and Crb2a^Extra_Secr (1.6 ± 0.06 μm) are similar to wildtypes. (D, D′) Area of the myoid region was outlined as in (D) and the measurements are shown in (D′). The area of the myoid region is significantly larger in Crb2a^FL (10.7 ± 0.5 μm²), Crb2a^Extra_TM (8.1 ± 0.4 μm²), and Crb2a^Extra_Secr (9.1 ± 0.5 μm²) compared to wildtypes (6.7 ± 0.2 μm²). Each circle represents a measurement of a single rod. Shown values are mean ± s.e.m, *, p < 0.005, student′s t test.