Transgenic Construct

Tg1(Xla.Rho:crb2a-HA)

ID

ZDB-TGCONSTRCT-110128-4

Previous Names

Extra_TM (1)
Tg(Xla.Rho.crb2.1-HA)
Tg(Xla.Rho:crb2.1-HA) (1)
Tg(Xla.Rho:crb2a.1-HA)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

crb2a (1)
HA (1)

Contains Sequences

HA (1)

Note

This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Extra_TM construct, site directed mutagenesis was used to place a Stop codon after the transmembrane domain.

Plasmid Map

None

Genomic Features

That Utilize Tg1(Xla.Rho:crb2a-HA) Construct

Genomic Feature	Affected Genomic Regions
umz4Tg
umz5Tg
umz6Tg

Transgenics

That Utilize Tg1(Xla.Rho:crb2a-HA) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
slc45a2^b4/+; fl1Tg/+; umz4Tg/+	slc45a2
slc45a2^b4/+; fl1Tg/+; umz5Tg/+	slc45a2
slc45a2^b4/b4; fl1Tg; umz4Tg	slc45a2	Fig. 4 from Hsu et al., 2010	6 figures from Hsu et al., 2010
slc45a2^b4/b4; fl1Tg; umz5Tg	slc45a2
slc45a2^b4/b4; fl1Tg; umz6Tg	slc45a2
umz6Tg

Sequence Information

Other Construct Pages

None

Citations