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FIGURE 4
sox7 and etv2 act cooperatively to rescue the hematopoietic defect of gfi1aa mutant (A, B) Expression of alas2 (A) and flk1 (B) in siblings and gfi1aa −/− mutants injected with 0.5 pmol sox7 MO, 0.005 pmol etv2 MO, 0.5 pmol sox7 MO with 0.005 pmol etv2 MO or control. The red arrows indicated WISH signals and the red boxes indicated the magnification of ICM region. Scale bar: 200 μm (C,D) Analysis of alas2 (C) and flk1 (D) expression in siblings and gfi1aa −/− mutants rescued by sox7 MO, etv2 MO and sox7 MO with etv2 MO. The asterisks indicate the statistical difference of the rescued proportion by MO compared to gfi1aa −/− (Three independent experiments were performed, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, t-test, n ≥ 10 embryos for each group).