IMAGE

Fig 2

ID
ZDB-IMAGE-211116-11
Genes
Antibodies
Source
Figures for Bühler et al., 2021
Image
Figure Caption

Fig 2

Early progenitor and chamber specification proceed normally in bal mutants.

(A-D) Whole-mount antisense RNA in situ hybridization (ISH) of cmlc1 and vmhc showed similar expression in bal mutants and wt siblings. (E, F) TUNEL staining (green) and IF for Mef2 (red) on dissected wt (E) and bal mutant hearts (F). (G) Quantification of TUNEL+ CMs revealed no alterations in apoptosis (wt 0.75 ± 0.70%, n = 4, bal 1.07 ± 1.28%, n = 6, p = 0.92), error bars indicate s.d., ns, not significant. (H, I) IF against Mef2 (red) and EdU (green) on dissected wt and bal hearts showed decreased numbers of EdU+ ventricular CMs. (J) Quantification revealed significantly decreased levels of EdU+ ventricular CMs in bal mutant hearts (wt 4.10 ± 2.73, n = 9, bal 0.61 ± 0.92, n = 11, p = 0.001). For significance testing Mann-Whitney-U test was applied, error bars indicate s.d., ***p < 0.001, ns, not significant.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.