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Figure 6 notch3, her4.1 and id1 are co-expressed in the NSCs of the telencephalon. (A–A’’) FISH against notch3 mRNA (A, red) on transverse sections through the telencephalon of Tg(id1-CRM2:gfp) transgenic animals (B, green). A’’ merged view of panels (A,A’). An overlapping pattern of expression for notch3 and Tg(id1-CRM2:gfp) (green) was noted in the telencephalon (white arrows). (B) Summary of co-expression analyses of Tg(id1-CRM2:gfp) with notch3. (C–C’’) Immunostaining on cross sections of the telencephalon of Tg(her4.1:gfp;id1-CRM2:mCherry) double transgenics with antibodies against GFP (green), and mCherry (red). GFP and mCherry signals co-localize indicating that her4.1 and id1 are co-expressed. (D) Summary of co-expression analyses of Tg(id1-CRM2-mCherry) with Tg(her4.1:gfp). n = 3 brains. Scale bar = 20 μm.