IMAGE

Fig. 4

ID
ZDB-IMAGE-180622-30
Genes
Source
Figures for Missinato et al., 2018
Image
Figure Caption

Fig. 4

dusp6 mutant hearts show accelerated regeneration. (A,B) Sections of hearts at 20 dpa stained with AFOG to visualize the deposition of fibrotic tissue (arrows). dusp6 mutant hearts (n=13) (B) have reduced fibrotic tissue area compared with WT hearts (n=17) (A). (C) Quantitation of fibrotic tissue area at 20 dpa. ***P<0.001, Student's t-test. (D) Representative ECG from WT (n=25) and dusp6 mutants (n=24) at 20 dpa. dusp6 mutant fish exhibit fewer arrhythmic events than WT. ECG are shown from four fish (#1, #10, #11, #13). (E) Quantitation of the arrhythmic events, measured as R-R intervals, in WT and dusp6pt30a/pt30a zebrafish at 20 dpa. ****P<0.0001, Student's t-test. (F) RNA-seq analysis of uninjured hearts and hearts at 20 dpa. At 20 dpa, WT hearts express fibrosis genes, but in dusp6pt30a hearts fibrosis genes are downregulated, suggesting faster regeneration. (G) Q-PCR analysis of selected fibrosis genes in hearts at 20 dpa. dusp6pt30a hearts show downregulation of fibrosis genes. Values are normalized to β-actin and rnap expression. Data are from one representative experiment from three independent biological replicates.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development