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Fig. 6
P53 mediates the regulation of Rpc9 in HSPC survival. (A) WISH result reveals that p53 is specifically increased in the CHT region of rpc9-/- embryos at 3 and 4dpf. (B) Western blot shows that, compared with rpc9+/+ embryos, the protein level of P53 was greatly increased in rpc9-/- embryos at 4dpf. (C) The quantitative result of the western blot in B. (D) The absence of rag1 in the thymus of rpc9-/- embryos, at 5dpf, can be rescued by loss of p53. (E) The absence of cmyb in the CHT region of rpc9-/- embryos at 5dpf can be rescued by p53 deficiency. (F) qRT-PCR result reveals that the decrease of cmyb in the CHT region of rpc9-/- embryos at 5dpf can be rescued by loss of p53. Note that arrowheads in A,E mark the CHT, whereas arrowheads in D mark the thymus. Values are mean±s.e.m. ***P<0.001, Student′s t-test.