Fig. 6

The axoneme is absent in pday mutant photoreceptors. (A-C) Wild-type and pday mutant retinas with anti-acetylated α-tubulin (red) and anti-Eys (green) antibodies (A); anti-γ-tubulin (red) and anti-Eys (green) antibodies (B); and anti-γ-tubulin (red) and anti-Cep290 (green) antibodies (C). (D) Wild-type and pday mutant retinas carrying the transgenes Tg[gnat2: arl13b-tdTomato] (red) and Tg[rho: ail13b-eGFP] (green). (E-Fʹ) Electron microscopy analysis of wild-type (E) and pday mutant (F) photoreceptors at 3 dpf. The bottom-left panels show a higher magnification (3000×) of the apical region of photoreceptors in wild-type siblings (E′) and pday mutants (F′). The bottom-right panels are enlarged images of the rectangular region shown in the bottom left panels. Red asterisks indicate the OS. Red and yellow arrowheads indicate the basal body and ciliary axoneme, respectively. The orange arrowhead indicates the short ciliary extension from the basal body observed in pday mutants. (G,H) Labeling of wild-type and pday mutant retinas with zpr3 (G) and 1D4 (H) antibodies. The open arrowheads indicate ectopic distribution of zpr3 and 1D4 signals in the plasma membrane including the synaptic area in pday mutants. In A-D,G,H, nuclei were counterstained with Hoechst 33342 (blue). In A-C, except for the mutant image in B, the bottom panels show a higher magnification of the ONL shown in the top panels (white boxes). Sample sizes are shown in Table S3. Scale bars: 5 µm (top panels of A-C; bottom panels of D,G,H); 1 µm (bottom panels of A-C; E-F′); 20 µm (top panels of D,G,H).