Figure 8—figure supplement 2.

Loss of rln3a NI neurons increases turning behavior.

Ratio of the total size, in degrees, of all calculated angles during the first 115 s of the recording, divided by total distance traveled in millimeters. (A) Mean ratio for unablated controls (81.94±12.85), or larvae with ablated gsc2 (81.53±15.81), rln3a NI (182.72±15.29), or rln3a PAG (84.26±16.70) neurons. Kruskal-Wallis rank sum test: ***p=0.00017. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated ***p=0.00038, ablated rln3a vs. gsc2 NI neurons ***p=0.00066, or ablated rln3a NI vs. rln3a PAG neurons ***p=0.00066. (B, C) Unablated control group includes only (B) Tg(gsc2:QF2)^c721; Tg(QUAS:GFP)^c578 or (C) Tg(rln3a:QF2, he1.1:YFP)^c836; Tg(QUAS:GFP)^c578 siblings of ablated larvae. Mean ratios: (B) Unablated c721=66.93±15.38. (C) Unablated c836=93.95±19.50. All other groups have the same values as in A. (B) Kruskal-Wallis rank sum test: ***p=0.000097. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated ***p=0.00057, ablated rln3a vs. gsc2 NI neurons ***p=0.00059, or ablated rln3a NI vs. rln3a PAG neurons ***p=0.00061. (C) Kruskal-Wallis rank sum test: ***p=0.00045. Dunn’s post-hoc tests with adjustment for multiple comparisons show ablated rln3a NI neurons vs. unablated **p=0.0045, ablated rln3a vs. gsc2 NI neurons **p=0.001, or ablated rln3a NI vs. rln3a PAG neurons **p=0.001.