Figure 5.

gsc2 and rln3a NI neurons innervate different dorsoventral IPN regions.

(A-H’) Confocal images of 6 dpf larvae. (A-B, E-F’) TgBAC(gng8:Eco.NfsB-2A-CAAX-GFP)^c375 and Tg(gsc2:QF2)^c721 driving (A, A’) Tg(QUAS:NLS-mApple, he1.1:CFP)^c718 or (B, E-F’) Tg(QUAS:mApple-CAAX, he1.1:mCherry)^c636. (C-D, G-H’) TgBAC(gng8:GAL4FF)^c426; Tg(UAS-E1B:NTR-mCherry)^c264 and Tg(rln3a:QF2, he1.1:YFP)^c836 driving (C, C’) Tg(QUAS:NLS-GFP, he1.1:CFP)^c682 or (D, G-H’) Tg(QUAS:NLS-GFP, he1.1:CFP)^c682 and Tg(QUAS:GFP-CAAX)^c591. (A’, C’) Higher magnification images of larvae in A and C, respectively. (A, A’, C, C’) Z-projections. (B, D) optical sections. (A–D) Lateral views, anterior left. (E-H’) Dorsal views, anterior to the top. Optical sections at the level of the (E, E’, G, G’) dorsal IPN or (F, F’, H, H’) ventral IPN of the same larvae. (E’, F’, G’, H’) Labeled efferent projections only. (I, J) Confocal Z-projections of coronal sections (70 μm) through adult brains of (I) Tg(gsc2:QF2)^c721; Tg(QUAS:GFP-CAAX; he1.1:YFP)^c631 or (J) Tg(rln3a:QF2; he1.1:YFP)^c836; Tg(QUAS:GFP-CAAX)^c591 fish. Anterior to the top. (K) Schematic of the IPN showing distinct dorsoventral regions innervated by rln3a and gsc2 neurons. Scale bars, 100 µm. dIPN: dorsal IPN, vIPN: ventral IPN.