Figure 7

The effect of s100a1 knockdown on the development of kras^G12V-induced HCC in zebrafish larvae. (A) Experiment design of s100a1 knockdown and oncogene induction in Lipan larvae and kras+ larvae. (B) Western blot of S100A1with the total protein extracted from MO-injected zebrafish embryos at 24 hpf. β-actin served as a loading control. Each protein sample was pooled from 15 zebrafish embryos. The quantification of the intensity of S100A1normalized by the intensity of β-actin was at the lower panel. The uncropped image of blots is shown in Figure S1. (C) Fluorescence images of morpholino-treated Lipan and kras+ larvae after 48 h of Dox treatment. (D) Measurement of liver size based on the fluorescence in (B) (n ≥ 8). Circles, squares, and triangles indicate values of individual samples (E) Expression of sox5, sox9a, and sox9b in the kras+ livers at 24 h after PH/sham surgery as determined by RT-qPCR (n = 3). Scale Bar = 200 μm. ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01.