FIGURE

FIGURE 1

ID

ZDB-FIG-240422-37

Publication

Gonzalez et al., 2024 - Knockdown of tgfb1a partially improves ALS phenotype in a transient zebrafish model

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FIGURE 1

Knockdown of tgfb1a partially prevents motor axon abnormalities in hSOD1^G93A-expressing zebrafish embryos. (A,B) Zebrafish embryos at the one-cell stage were subjected to microinjection with hSOD1^WT mRNA (n = 10), hSOD1^G93A mRNA (n = 12), tgfb1a-MO (n = 8), or co-microinjected with hSOD1^G93A mRNA and hSOD1^G93A mRNA (n = 9). Additionally, a non-injected group (n = 12) served as a control. At 48 hpf, the embryos were immunostained with Znp-1 antibody, and motor axon morphology was analyzed using ImageJ software with NeuronJ plugin, as previously described (Meijering et al., 2004; Robinson et al., 2019). The graphs depict quantifications of (C) axon length and (D) total length of motor axons (5–7 motor axons per embryo). Box and whisker plots represent the 25th and 75th percentiles, with medians represented by bisecting lines and means denoted by “+.” Whiskers indicate extreme values. Statistical differences were assessed using one-way ANOVA and Tukey’s multiple comparison test, *p < 0.05, **p < 0.01, n.s.: not significant. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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