Fig. 6

Differential transcriptomic regulation of genes expressed in both developing and regenerating zebrafish epicardia (A and B) Network connection scores within the network representing the developing epicardium (A) and that representing the regenerating epicardium (B) between selected TFs and genes expressed in both conditions. (C) TF motifs within the mdka_e1 sequence. (D) TF motifs within the mdka_e2 sequence. Representative accessibility of the endogenous genomic region in tcf21 larval epi (C) or in tcf21 cryo (D) is underlaid in gray. Bubble size indicates the eigenvector centrality of the factor in the tcf21 larval epi sub-network (C) or the tcf21 cryo sub-network (D). (E) mRNA staining of klf9 (magenta) and tcf21 (green) in a 5-dpf heart. (E′ and E′′) A nucleus (asterisk) in the epicardial region surrounded by klf9 and tcf21 transcripts. (F) mRNA staining of tbx2b (magenta) and tcf21 (green) in a 3-dpi cryoinjured adult heart. Ia, injury area. (F′ and F′′) A single nucleus (asterisk) in the epicardial region surrounded by tbx2b and tcf21 transcripts. (G) Predicted upstream transcriptional regulation in the developing epicardium. (H) Upstream regulation in the regenerating epicardium. In (G) and (H), all interactions are activating. Scale bars: 100 μm in (F); 20 μm in (E); and 5 μm in (E′), (E′′), (F′), and (F′′). Color channels have been adjusted separately for brightness/contrast. (E) and (F) are single optical sections. TFBS, transcription factor binding sites; V, ventricle. See also Figure S5.