FIGURE 4

Detachment of CNH cells in mastl^kt441b homozygous embryos. (A) The experimental design. mastl^kt441b heterozygous male and female fish harboring transgene Bac(foxa2:Kaede) were crossed, and CNH cells of the progeny were photoconverted at the 4 to 5-somite stage. Five and half to 6 h after photoconversion, pictures of embryos were taken. Two hypotheses depicted in the figure can be distinguished by examining whether photoconverted (judged by red fluorescence) Kaede still remains in detached cells. (B) Examples of photoconverted tails. Pink arrows indicate Kaede-positive cells located outside the axial tissue observed at 5.5–6 h after photoconversion. Scale bar, 50 μm. (C) Kaede-positive cells outside of the CNH at 5.5–6 h after photoconversion were counted. The total number of cells of 3 embryos in the siblings or mastl^kt441b homozygous mutant are shown.