Fig. S4

Cold treatment slows development and reveals cell-type-specific temperature-responsive genes in the normal range of zebrafish development, related to Figure 3 (A) Dot plot showing the sampling timing for cold-treated embryos (x axis is clock time), as well as their predicted whole-embryo stage based on integration with the 28°C reference data; the predicted stage roughly matched the expectation of the Kimmel et al.24 (B) Boxplot showing the effect of 5 μM CHX on temperature-induced acceleration of whole-embryo stage; developmental delay is evident in CHX-treated embryos raised at 28°C, and this effect is not overridden by 34°C temperature treatment. (C) Scatterplot showing temperature-dependent activation of genes of the fast muscle, expressed as model coefficients for each temperature after accounting for developmental stage. Most genes showed either heat-induced increases and cold-induced decreases in expression (red box in upper left) or heat-induced decreases and cold-induced increases in expression (blue box in lower left). (D) Scatterplot as in (C), but for slow muscle. € Scatterplot for fast muscle similar to (B) but showing cold vs. CHX treatment, which shows an overall strong positive correlation with gene expression similarly affected by both sources of developmental delay. (F) Scatterplot as €(E), but for slow muscle.