Fig. 3

Temperature introduces asynchrony in developmental rate across cell types (A) Dot plots showing the transcriptional ages of all cell types at 24 hpf, faceted by temperature. Cell types on the y axis are ordered by their relative acceleration at 34°C, highlighting the most sensitive cell types near the top and insensitive types near the bottom. Cell type ordering is the same for all temperatures; specific examples are indicated with labels. (B) Histogram showing distributions of relative cell type progression at each temperature, with vertical lines showing the relative progression expected for the whole embryo. (C) Bar plot showing the effect sizes for expression levels of several cellular processes related to metabolism, protein folding, proliferation, and stress response in an additive model predicting relative progression of the cell type at high temperature. (D) Scatterplot showing basal levels of the UPR in each cell type against its relative progression, revealing a positive trend for both temperatures. (E) Density histogram showing increased variance in developmental stage for embryos raised at elevated temperature. (F) Heatmaps showing pairwise correlation coefficients of transcriptional age for all cell types in the embryo; at 28°C, most cell type pairs are positively correlated across individual embryos, whereas this correlation structure is diminished at elevated temperatures. (G) Density histogram of pairwise correlation values at each temperature, summarizing the loss in correlation structure seen in (F).