FIGURE

Fig. 4

ID
ZDB-FIG-231227-33
Publication
Greysson-Wong et al., 2023 - rasa1-related arteriovenous malformation is driven by aberrant venous signalling
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Modulation of flow does not rescue rasa1−/− malformations. (A-F) Confocal images of wild type and rasa1−/− on Tg(kdrl:EGFP) (black) when treated with DMSO, isoprenaline (iso) and phenylhydrazine (PTZ). Vascular malformations are outlined with a red dashed line. (G) Quantification of dorsal aorta diameter shows no significant difference between wild types and rasa1−/− with either drug [WTDMSO (n=13) versus rasa1−/−DMSO (n=9), P=0.19; WTiso versus rasa1−/−iso (n=6), P=0.88; WTPTZ versus rasa1−/−PTZ (n=10), P=0.74; N=3 experiments]. (H) Plexus outgrowth in wild type and rasa1−/− is significantly larger than wild types (WTDMSO versus rasa1−/−DMSO, P=0.21; WTiso versus rasa1−/−iso, P<0.0001; WTPTZ versus rasa1−/−PTZ, P<0.0001; N=3 experiments). Isoprenaline appears to increase plexus outgrowth when compared with untreated rasa1−/−DMSO (P=0.0086). (I) Increased largest vein diameter is not rescued in rasa1−/− upon either increasing or decreasing blood flow (WTDMSO versus rasa1−/−DMSO, P<0.0002; WTiso versus rasa1−/−iso, P<0.0001; WTPTZ versus rasa1−/−PTZ, P<0.0001, N=3 experiments). (J-M) Confocal images of wild type and rasa1−/− on Tg(kdrl:EGFP) (black) when treated with DMSO and nifedipine (nif). No treatments rescue rasa1−/− malformations. (N) Heart rate of wild-type embryos treated with isoprenaline increased by 6.6% [wild type, 177.1 bpm (n=14); WTiso, 114.2 bpm (n=16), P=0.0024; N=4 experiments]. (O) Heart rate of wild-type embryos treated with nifedipine decreased by 11.2% [WTDMSO, 117.7 bpm (n=20); WTnif, 104.5 bpm (n=23); P=0.0074; N=4 experiments]. (P) Quantification of largest vein diameter in wild types and rasa1−/− treated with DMSO and nifedipine [WTDMSO (n=12) versus rasa1−/−DMSO (n=3); P<0.0001; WTnif (n=13) versus rasa1−/−nif (n=6); P<0.0001, N=4 experiments]. Statistical analysis was carried out using one-way ANOVA with Sidak's correction for drug experiments. An unpaired t-test was used for heart rate analysis. Data are mean±s.d. Scale bars: 20 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development