FIGURE

Figure 4

ID

ZDB-FIG-231130-69

Publication

Sun et al., 2023 - Isolation and proteomic study of fish liver lipid droplets

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Figure 4

Isolation of LDs from Carassius auratus liver. A Livers of Carassius auratus were collected and homogenized by dounce, the fraction of PNS was collected after centrifugation at 500 g and subjected to separation of LD fraction. After centrifugation with the indicated speed, the white layer on the top of the gradient was collected as LD fraction, the pellet at the bottom was resuspended as TM, and the middle solution was collected as Cyto. The LD fraction was then washed three times and kept in an Eppendorf tube as isolated LDs for further usage. B Isolated LDs were first analyzed by confocal microscopy with LipidTOX Red staining, DIC imaging, and merged images. Bar = 10 μm. C The size of isolated LDs was measured by a Delsa Nano C particle analyzer

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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