Fig. 2

The effect of SVCV on zebrafish gene transcription is eliminated by GCRV. A Schematic representation of zebrafish tissue dissection and RNA extraction for transcriptome sequencing. The liver and spleen tissues from male and female zebrafish injected with PBS (10 μL/individual), SVCV [5 × 10⁹ 50% tissue culture infective dose (TCID₅₀)/mL, 5 μL/individual and 5 μL PBS to make up 10 μL/individual] and SVCV&GCRV (5 × 10⁷ TCID₅₀/mL, 5 μL/individual) for 72 h. Total RNAs were extracted and used for transcriptome sequencing and analysis. B Heat maps of representative gene modules of STEM analysis in different tissues. The P-values were corrected for the False Discovery Rate (FDR) method, and modules with P-values less than 0.05 were selected as significant. C Quantitative PCR (qPCR) validates changes in representative genes of immune system. The β-actin gene was used as an internal control, and the relative expressions of ifn and irf3 genes were represented as fold induction relative to the expression level in control cells (set to 1). Data were expressed as mean ± standard error of the mean (SEM), n = 3. Statistical analysis was performed by the Student's t-test. Asterisks indicate significant differences from control (^∗P < 0.05). D GSEA enrichment plots in SVCV versus Null and SVCV&GCRV versus SVCV groups. E Rose plots showing the number of DEGs at SVCV versus Null and at SVCV&GCRV versus SVCV in selected pathways (FC > 1.5, adjusted P < 0.05).