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Fig. 1

ID

ZDB-FIG-230712-8

Publication

Siddiqui et al., 2023 - Nerve growth factor receptor (Ngfr) induces neurogenic plasticity by suppressing reactive astroglial Lcn2/Slc22a17 signaling in Alzheimer's disease

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Fig. 1

NGFR enhances proliferative and neurogenic markers in dentate gyrus (DG) astrocytes.

a Schematic strategy for expression lentiviruses: Lv13: mCherry control; Lv16: Ngfr-mCherry. b Cross section images for DGs transduced with Lv13 and Lv16 and immunostained for mCherry, NGFR with DAPI counterstain. Note NGFR expression in the subgranular zone (SGZ) after Lv16 transduction. c Single cell transcriptomics tSNE plot from dissected DGs after Lv13 and Lv16 transduction. d tSNE plots indicating cell types. e Number of cells sequenced and their distribution to individual cell types. f tSNE plots from Lv13 and Lv16 transduction showing the expression of Ngfr-mCherry, which is detectable only after Lv16. g KEGG pathway analyses on astrocytic population, showing downregulated and upregulated pathways. h A heat map for selected differentially expressed genes (DEGs) after Lv16. Astrocyte markers are downregulated, and proliferation/neurogenesis markers are upregulated. i Violin plots for selected genes: purple: control (Lv13); yellow (Lv16). j Immunostaining for validating the DEGs in control and Lv16-transduced DG. Scale bars equal 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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