FIGURE

FIGURE 5

ID

ZDB-FIG-230228-110

Publication

Boyd et al., 2023 - Clcf1/Crlf1a-mediated signaling is neuroprotective and required for Müller glia proliferation in the light-damaged zebrafish retina

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FIGURE 5

Knockdown of CNTFR and Clcf1/Crlf1a reduces Müller glia proliferation. (A–H) Single confocal images from dark-adapted albino; Tg[gfap:EGFP] ^nt11 zebrafish which were light-treated for 36 h after electroporation of either S.C. (A), cntfr (B), clcf1/crlf1a morpholinos (C), or a combination of all three cntfr/clcf1/crlf1a (Triple) (D). Sections were labeled for GFP and PCNA to assess proliferating Müller glia and nuclei were counterstained with DAPI (E, F, G, H). (I) Quantification showing significant decrease in the number of PCNA-positive Müller glia within the INL in cntfr and clcf1/crlf1a electroporated retinas. One-way ANOVA with Dunnett post hoc, cntfr vs. S.C. p < 0.0001, clcf1/crlf1a vs. S.C. p = 0.0003, Triple vs. S.C. p = 0.0072. n ≥ 12. Mean ± SEM, **p < 0.001, ***p < 0.001, ****p < 0.0001. ONL, outer nuclear layer, INL, inner nuclear layer, GCL, ganglion cell layer. Scale bar represents 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol